Primer in Clinical Proteomics
Presented by
Duncan Lab
Department of Pediatrics
University of Colorado School of Medicine
Sponsored by
University of Colorado School of Medicine
Office of Continuing Medical Education
COURSE DATE 2008: Thursday May 8
Cost: $95
We now offer this course several times per year and it is open to UCDHSC staff and students, as well as attendees from other institutions.
Clinical Proteomics is an emerging field that seeks to identify new biomarkers useful for the diagnosis of disease or monitoring of patient response to therapy. However, the methodological, analytical, and statistical challenges facing clinical researchers can make success difficult to achieve. This course will prepare clinical researchers to design a successful proteomics study, while exposing them to the potential pitfalls in the multi-dimensional nature of biomarker discovery.
In this one-day lecture course, students will gain an appreciation for the challenging aspects of biomarker discovery projects, as well as to the potential solutions for each of these hurdles. Course topics include: Study design and sample collection/storage of clinical samples; Sample handling and preparation; gel-based proteomics including DIGE; MALDI-TOF, Multiplexed protein and cytokine arrays, Quantitative data analysis of 2D and Mass Spectrometry data sets, Quantitative post-discovery validation methods (Westerns, ELISAs).
Clinical proteomics is a field that utilizes knowledge from many disciplines, so this course is taught by experts in comparative proteomics, biochemistry, analytical chemistry, mass spectrometry, bioinformatics and clinical medicine.
Upon completion of this program:
- Describe the workflow of several common proteomic strategies used in a clinical proteomics collaboration.
- Be able to identify pitfalls in the design of a clinical proteomics study.
- Gain strategies for overcoming limitations of commonly used proteomic techniques.
- Gain confidence and skills to plan a successful clinical proteomics experiment.
Mark Duncan Ph.D., Stephen Hunsucker Ph.D. and Heather Thompson Ph.D.
**Please indicate if you have any need for auxiliary aids or special assistance services.
Accreditation:
The University of Colorado School of Medicine is accredited by the Accreditation Council for Continuing Medical Education (ACCME) to provide continuing medical education for physicians.
Credit Designation:
The University of Colorado School of Medicine designates this educational activity for a maximum of (5.25) AMA PRA Category 1 Credit(s)™. Physicians should only claim credit commensurate with the extent of their participation in the activity.
This course supported in part by a Clinical Research U01 from the NHLBI.
This program is a part of the Colorado Statewide Extended Campus. State funds were not used to develop or administer this program.
Space is limited, so register now!
If you have questions. please contact James Simpson
Hands-on Practical Proteomics Short Course
COURSE DATES 2008: TBA
Cost: $995
We now offer this course once per year and it ilease indicate if you have any need for auxiliary aids or special assistance services. Accreditation: The University of Colorado School of Medicine is accredited by the Accreditation Council for Continuing Medical Education (ACCME) to provide continuing medical education for physicians. Credit Designation: The University of Colorado School of Medicine designates this educational activity for a maximum of (number of credits) AMA PRA Category 1 Credit(s)™. Physicians should only claim credit commensurate with the extent of their participation in the activity. This course supported in part by a Clinical Research U01 from the NHLBI. This program is a part of the Colorado Statewide Extended Campus. State funds were not used to develop or administer this program.s open to UCDHSC staff and students, as well as attendees from other institutions. This intensive, four-day training course is designed for anyone wanting to gain hands-on proteomics experience. The course includes lectures on the theory and practice of all relevant techniques, and the afternoon laboratory session provides an opportunity to put this knowledge into practice. Attendees will also receive a detailed set of course notes incorporating background reading material, detailed methods and self-assessment exercises. Practical Proteomics provides a unique balance of practical, laboratory-based and tutorial-style classroom sessions. Participants will be exposed to the entire range of techniques, including 2D gel electrophoresis, MALDI and electrospray MS, tandem mass spectrometry, and database searching of mass spectral data. The course is taught by expert practitioners in proteomics, including clinicians from the UCHSC campus. Course content includes discussion of the following:
- Study Design
- Sample Preparation and Protein Isolation
- Tissues
- Body fluids
- Cultured cells
- 2D Gel Electrophoresis
- Isoelectric focusing (1st dimension)
- SDS PAGE (2nd dimension)
- Staining and visualization
- Quantitative image analysis
- Mass Spectrometry of Peptides and Proteins
- In-gel tryptic digestion
- MALDI-ToF MS
- LC/MS/MS
- Comparative Proteomics
- Difference Gel Electrophoresis (DIGE)
- Isotopic Methods (iTRAQ, ICAT)
- Bioinformatics
- Peptide mass fingerprinting
- Approaches to dealing with MS/MS data
- Useful tools in proteomics
- Limitations and Pitfalls in Proteomic Analyses
